Construction of the pEGFP-N1-p53/MAR Vector and Its Effect on HEP3B Cell Morphology
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چکیده
منابع مشابه
an investigation of the impact of self monitoring on langauge teachers motivational practice and its effect on learners motivation
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15 صفحه اولConstruction of the mammalian expressing vector pEGFP-N1-P53 and its expression successful in chicken fibroblast cells and blastoderm.
The enhanced green fluorescent protein (EGFP) pEGFP-N1-P53 eukaryotic expression vector, which contains the human tumor suppressor p53, was constructed and transfected into chicken fibroblast cells and stage-X blastoderm to analyze the transfection efficiency. The complementary DNA of the human p53 gene was cloned by reverse transcription-polymerase chain reaction from human peripheral blood an...
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15 صفحه اولConstruction of recombinant pEGFP-N1-hPer2 plasmid and its expression in osteosarcoma cells
The aim of this study was to construct the eukaryotic expression vector pEGFP-N1-hPer2 and assess its expression in the human osteosarcoma cell line MG63. Total mRNA was extracted from human osteosarcoma MG63 cells, the human period 2 (hPer2) gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into the pEGFP-N1 vector, then the recombinant pEGFP-N1-hPer2 pla...
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ژورنال
عنوان ژورنال: Sains Malaysiana
سال: 2018
ISSN: 0126-6039
DOI: 10.17576/jsm-2018-4709-19